Intermediate

Part:BBa_M1210:Design

Designed by: Cameron Copeland   Group: UtahState_BE5930_-_S11   (2011-04-22)


Tet Repressor/Promoter with ROSE Regulation and Kill Gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2939
    Illegal NheI site found at 2962
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2544
    Illegal AgeI site found at 2614
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1505
    Illegal SapI site found at 3215


Design Notes

This gene is great, but not as great as others we've seen or heard about. It is average greatness, definitely worth an A but not necessarily extra credit worthy.


Source

http://2010.igem.org/Team:HKU-Hong_Kong

References